1528 pET28b.Amp.HC
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Cloning of 1528 pET28b.Amp.HC
[edit]Purpose: Cloning of 1528 pET28b.Amp.HC
Date:Dec, 2012
Notebook:
Page:
Clone ID: 1528
Experimenters: shanyi
Results
[edit]Cloning
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digest pET28b with DraIII + Sap1, --> vector digest pRestA with DraIII+ Sap1--> 2171 bp fragment.
Colony Screening
[edit]digest with BamHI and BsaI
Use sample1 .
1528-1 digest with ScaI + SapI
puc19 digest with ScaI + SapI+NdeI
Result intepretation/Summary
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Conclusion
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