1805-pCold-MCS-pLacZ-3C

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Cloning of 1805-pCold-MCS-pLacZ-3C

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Purpose: Cloning of 1805-pCold-MCS-pLacZ-3C

Date: 2013-8-7---2013-8-14

Notebook:0

Page:

Clone ID:1805

Experimenters: yingying

Results

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cloning

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PCR lacZ promoter using pUC19 as template.
LacZ-Pro-BamH1-F
LacZ-Pro-3C-R


3C protease from 1420 pDB.His.MBP.3C
LacZ-Pro-3C-F
3C-Sph1-R


pcr1+pcr2

LacZ-Pro-BamH1-F+3C-Sph1-R



lane
 1
 2

 PCR product
 1755 pCold-6H
enzymes
 BamH1+Sph1
 BglII + Sph1


Colony Screening

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Date: 2013-8-14

Make miniprep and digest  Sph1+Spe1



Result intepretation/Summary

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Conclusion

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