1805-pCold-MCS-pLacZ-3C
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Cloning of 1805-pCold-MCS-pLacZ-3C
[edit]Purpose: Cloning of 1805-pCold-MCS-pLacZ-3C
Date: 2013-8-7---2013-8-14
Notebook:0
Page:
Clone ID:1805
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR lacZ promoter using pUC19 as template.
LacZ-Pro-BamH1-F
LacZ-Pro-3C-R
3C protease from 1420 pDB.His.MBP.3C
LacZ-Pro-3C-F
3C-Sph1-R
pcr1+pcr2
LacZ-Pro-BamH1-F+3C-Sph1-R
| lane |
1 |
2 |
| PCR product |
1755 pCold-6H | |
| enzymes |
BamH1+Sph1 |
BglII + Sph1 |
Colony Screening
[edit]Date: 2013-8-14
Make miniprep and digest Sph1+Spe1
Result intepretation/Summary
[edit]Conclusion
[edit]