1818-pT7A30-TthDNALigase

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Cloning of 1818-pT7A30-TthDNALigase

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Purpose: Cloning of 1818-pT7A30-TthDNALigase

Date: 2013-8-13-----2013-8-16

Notebook:0

Page:

Clone ID:1818

Experimenters: yingying

Results

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cloning

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lane
 1
 2

 1035, pET28b-Tth-Ligase-K294R
 1727 pT7A30
enzymes
               Nde1 + EcoR1


Colony Screening

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Date: 2013-8-16

Make miniprep and digest with Nde1 +BamH1/ BamH1+EcoR1



clone 2 digest with Nco1+Hind111

clone 2 for stock


Result intepretation/Summary

Conclusion

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