1818-pT7A30-TthDNALigase
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Cloning of 1818-pT7A30-TthDNALigase
[edit]Purpose: Cloning of 1818-pT7A30-TthDNALigase
Date: 2013-8-13-----2013-8-16
Notebook:0
Page:
Clone ID:1818
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
1 |
2 |
| 1035, pET28b-Tth-Ligase-K294R |
1727 pT7A30 | |
| enzymes |
Nde1 + EcoR1 | |
Colony Screening
[edit]Date: 2013-8-16
Make miniprep and digest with Nde1 +BamH1/ BamH1+EcoR1
clone 2 digest with Nco1+Hind111
clone 2 for stock
Result intepretation/Summary
Conclusion
[edit]