1793-pET28b-VPol1
Cloning of 1793-pET28b-VPol1
[edit]Purpose: Cloning of 1793-pET28b-VPol1
Date: 2013-8-1----2013-8-7
Notebook:0
Page:
Clone ID:1793
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
VPol1-1-1 to VPol1-1-18
VPol1-2-1 to VPol1-1-2-14
VPol1-3-1 to VPol1-3-16
template:pcr1+pcr2+pcr3
VPol1-1-1+VPol1-3-16
| lane |
3 |
4 |
| PCR product |
1074,pET28b-IHFab | |
| enzymes |
Nco1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-7
Make miniprep and digest with Nco1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]
Fixing of Cloning of 1793-pET28b-VPol1
[edit]Purpose: fixing of Cloning of 1793-pET28b-VPol1
Date: 2013-8-14----2013-8-16
Notebook:0
Page:
Clone ID:1793
Experimenters: yingying
Results
[edit]cloning
[edit]
Template: 1793-2 (2013/8/7)
PCR1:1-1 to 2-4
PCR2:2-5 to 3-16
template:pcr1+pcr2
VPol1-1-1+VPol1-3-16
| lane |
1 |
2 |
| PCR product |
1074,pET28b-IHFab | |
| enzymes |
Nco1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-16
Make miniprep and digest with Nco1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]
2013-8-30
