1847-pT7A30-sso7d-6H

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Cloning of 1847-pT7A30-sso7d-6H

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Purpose: Cloning of 1847-pT7A30-sso7d-6H

Date: 2013-9-17---2013-9-20

Notebook:0

Page:

Clone ID:1847

Experimenters: yingying

Results

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cloning

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PCR using 1734 pT7A30-sso7d-KX  as template
Sso7d-Not1-F
sso7d-Sal1-R

lane
 3
 4

 PCR product
 1727 pT7A30

 Not1 + Sal1
 Not1 + Xho1


Colony Screening

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Date: 2013-9-20

Make miniprep and digest with Kpn1



Result intepretation/Summary

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Conclusion

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