1847-pT7A30-sso7d-6H
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Cloning of 1847-pT7A30-sso7d-6H
[edit]Purpose: Cloning of 1847-pT7A30-sso7d-6H
Date: 2013-9-17---2013-9-20
Notebook:0
Page:
Clone ID:1847
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1734 pT7A30-sso7d-KX as template
Sso7d-Not1-F
sso7d-Sal1-R
| lane |
3 |
4 |
| PCR product |
1727 pT7A30 | |
| Not1 + Sal1 |
Not1 + Xho1 |
Colony Screening
[edit]Date: 2013-9-20
Make miniprep and digest with Kpn1
Result intepretation/Summary
[edit]Conclusion
[edit]