673-pEC-mNhe1

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Cloning of 673-pEC-mNhe1

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Purpose: Cloning of 673-pEC-mNhe1

Date: 2013-9-16---2013-9-20

Notebook:0

Page:

Clone ID:673

Experimenters: yingying

Results

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cloning

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gene synthesis

pcr1:mNhe1-1-1 to  mNhe1-1-14

pcr2:mNhe1-2-1  to mNhe1-2-16



PCR: pcr1+pcr2

mNhe1-1-1 +mNhe1-2-16



lane
 3
 4

 PCR product
 1454,pEC

 HindIII + BamH1


Colony Screening

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Date: 2013-9-20

Make miniprep and digest with  HindIII + BamH1




Result intepretation/Summary

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Conclusion

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