673-pEC-mNhe1
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Cloning of 673-pEC-mNhe1
[edit]Purpose: Cloning of 673-pEC-mNhe1
Date: 2013-9-16---2013-9-20
Notebook:0
Page:
Clone ID:673
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
pcr1:mNhe1-1-1 to mNhe1-1-14
pcr2:mNhe1-2-1 to mNhe1-2-16
PCR: pcr1+pcr2
mNhe1-1-1 +mNhe1-2-16
| lane |
3 |
4 |
| PCR product |
1454,pEC | |
| HindIII + BamH1 | ||
Colony Screening
[edit]Date: 2013-9-20
Make miniprep and digest with HindIII + BamH1
Result intepretation/Summary
[edit]Conclusion
[edit]