1870-pET28b-HTNFlp-ecoli

From ELN
Revision as of 11:17, 21 October 2013 by Paul.xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 1870-pET28b-HTNFlp-ecoli

[edit]

Purpose: Cloning of 1870-pET28b-HTNFlp-ecoli

Date: 2013-10-16---2013-10-18

Notebook:0

Page:

Clone ID:1870

Experimenters: yingying

Results

[edit]

cloning

[edit]


PCR using 1812 pET28b-HNFlp-Ecoli as template
pcr 1
ecoliFLP-HindIII-F
ecoliFLP-R

pcr 2

ecoliFLP-F
Ecoli-Flpe-2-18

PCR
ecoliFLP-HindIII-F
Ecoli-Flpe-2-18


Lane
 5
 6

 PCR
 375 pET28b-Cre

 HindIII + Xho1


Colony Screening

[edit]

Date: 2013-10-18

Make miniprep and digest with Hind111+EcoR1



Result intepretation/Summary

[edit]

Conclusion

[edit]


Retrieved from "https://eln.excellgen.com/index.php?title=1870-pET28b-HTNFlp-ecoli&oldid=10443"