1870-pET28b-HTNFlp-ecoli
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Cloning of 1870-pET28b-HTNFlp-ecoli
[edit]Purpose: Cloning of 1870-pET28b-HTNFlp-ecoli
Date: 2013-10-16---2013-10-18
Notebook:0
Page:
Clone ID:1870
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1812 pET28b-HNFlp-Ecoli as template
pcr 1
ecoliFLP-HindIII-F
ecoliFLP-R
pcr 2
ecoliFLP-F
Ecoli-Flpe-2-18
PCR
ecoliFLP-HindIII-F
Ecoli-Flpe-2-18
| Lane |
5 |
6 |
| PCR |
375 pET28b-Cre | |
| HindIII + Xho1 | ||
Colony Screening
[edit]Date: 2013-10-18
Make miniprep and digest with Hind111+EcoR1
Result intepretation/Summary
[edit]Conclusion
[edit]