1879-pLysN-PampR-mNhe1

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Cloning of 1879-pLysN-PampR-mNhe1

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Purpose: Cloning of 1879-pLysN-PampR-mNhe1

Date: 2013-10-25---2013-10-28

Notebook:0

Page:

Clone ID:1879

Experimenters: yingying

Results

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cloning

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PCR AmpR promoter using pUC19 plasmid
AmpR-pro-HindIII-F
AmpR-pro-Nde1-R


lane
 1
 2

 PCR product
 674 pLysN-mNhe1

            HindIII + Nde1


Colony Screening

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Date: 2013-10-28

Make miniprep and digest with HindIII + Nde1




Result intepretation/Summary

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Conclusion

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