1879-pLysN-PampR-mNhe1
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Cloning of 1879-pLysN-PampR-mNhe1
[edit]Purpose: Cloning of 1879-pLysN-PampR-mNhe1
Date: 2013-10-25---2013-10-28
Notebook:0
Page:
Clone ID:1879
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR AmpR promoter using pUC19 plasmid
AmpR-pro-HindIII-F
AmpR-pro-Nde1-R
| lane |
1 |
2 |
| PCR product |
674 pLysN-mNhe1 | |
| HindIII + Nde1 | ||
Colony Screening
[edit]Date: 2013-10-28
Make miniprep and digest with HindIII + Nde1
Result intepretation/Summary
[edit]Conclusion
[edit]