1883-pT5

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Cloning of 1883-pT5

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Purpose: Cloning of 1883-pT5

Date: 2013-10-23---2013-10-28

Notebook:0

Page:

Clone ID:1883

Experimenters: yingying

Results

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cloning

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PCR using pQE-80 L  as template
primers:
T5-BglII-F
T5-Nde1-R


lane
 3
 4

 PCR product
 pET28b

       BglII + Nde1


Colony Screening

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Date: 2013-10-28

Make miniprep and digest with BglII + Nde1



digest with Xba1+Sma1

c:pET28b



Result intepretation/Summary

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Conclusion

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