1883-pT5
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Cloning of 1883-pT5
[edit]Purpose: Cloning of 1883-pT5
Date: 2013-10-23---2013-10-28
Notebook:0
Page:
Clone ID:1883
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using pQE-80 L as template
primers:
T5-BglII-F
T5-Nde1-R
| lane |
3 |
4 |
| PCR product |
pET28b | |
| BglII + Nde1 | ||
Colony Screening
[edit]Date: 2013-10-28
Make miniprep and digest with BglII + Nde1
digest with Xba1+Sma1
c:pET28b
Result intepretation/Summary
[edit]Conclusion
[edit]