1893 pPinkalpha-HC-Monellin-Y

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Cloning of 1893 pPinkalpha-HC-Monellin-Y

Purpose: Cloning of 1893 pPinkalpha-HC-Monellin-Y

Date: 2013-10-31---

Notebook:0

Page:

Clone ID:1893

Experimenters: shanyi

Results

PCR

pcr using 655 as template

Monellin-Kpn1-R tgctagGGTACCTTATGGTGGTGGAACTGGAC
Monellin-Xho1-F2 gatcga ctcgagaaa agg GGTGAGTGGGAGATTATTG

digest with KpnI+XhoI

Colony Screening

Date: 2013-11-1

Make miniprep, digest with KpnI+XhoI

send 1893-2 to sequence

Result intepretation/Summary

Conclusion

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Cloning