1893 pPinkalpha-HC-Monellin-Y

From ELN
Jump to navigation Jump to search

Cloning of 1893 pPinkalpha-HC-Monellin-Y

[edit]

Purpose: Cloning of 1893 pPinkalpha-HC-Monellin-Y

Date: 2013-10-31---

Notebook:0

Page:

Clone ID:1893

Experimenters:  shanyi

Results

[edit]

PCR

[edit]

pcr using 655 as template

Monellin-Kpn1-R tgctagGGTACCTTATGGTGGTGGAACTGGAC
Monellin-Xho1-F2 gatcga ctcgagaaa agg GGTGAGTGGGAGATTATTG


digest with KpnI+XhoI



Colony Screening

[edit]

Date: 2013-11-1

Make miniprep, digest with KpnI+XhoI


send 1893-2 to sequence



Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=1893_pPinkalpha-HC-Monellin-Y&oldid=10567"