1955-pLVX-Kozak-loxP-TurboRFP-ODC-2Stops-LoxP-TurboGFP

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Cloning of 1955-pLVX-Kozak-loxP-TurboRFP-ODC-2Stops-LoxP-TurboGFP

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Purpose: Cloning of 1955-pLVX-Kozak-loxP-TurboRFP-ODC-2Stops-LoxP-TurboGFP

Date: 2013-11-24----2013-11-27

Notebook:0

Page:

Clone ID:1955

Experimenters: yingying

Results

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cloning

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PCR1 BGH terminator from 1522 CMV-Brainbow-2.1-R using
BGHpA-EcoR1-F
BGHpA-hGHpA-R

2. PCR hGH terminator from 1427 pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP using
BGHpA-hGHpA-F
LoxP-hGHpA-Xho1-R2



LANE
 1
 2

 PCR
1427 pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP


             EcoR1 + Xho1


Colony Screening

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Date: 2013-11-27

Make miniprep and digest with EcoR1 + Xho1



Result intepretation/Summary

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Conclusion

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