1955-pLVX-Kozak-loxP-TurboRFP-ODC-2Stops-LoxP-TurboGFP
Jump to navigation
Jump to search
Cloning of 1955-pLVX-Kozak-loxP-TurboRFP-ODC-2Stops-LoxP-TurboGFP
[edit]Purpose: Cloning of 1955-pLVX-Kozak-loxP-TurboRFP-ODC-2Stops-LoxP-TurboGFP
Date: 2013-11-24----2013-11-27
Notebook:0
Page:
Clone ID:1955
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR1 BGH terminator from 1522 CMV-Brainbow-2.1-R using
BGHpA-EcoR1-F
BGHpA-hGHpA-R
2. PCR hGH terminator from 1427 pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP using
BGHpA-hGHpA-F
LoxP-hGHpA-Xho1-R2
| LANE |
1 |
2 |
| PCR |
1427 pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP | |
| EcoR1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-11-27
Make miniprep and digest with EcoR1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]