718-pT7A3E-wt-BamH

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Cloning of 718-pT7A3E-wt-BamH

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Purpose: Cloning of 718-pT7A3E-wt-BamH

Date: 2013-12-6---2013-12-19

Notebook:0

Page:

Clone ID:718

Experimenters: yingying

Results

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cloning

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Wt-BamH1-Nde1-F ctagcacatatggaagtagaaaaagagttta
Wt-BamH1-Xho1-R ttgctagctcgagtttgttttcaactttatctttc
PCR from 712 ptrc-BamHIR


lane
 3
 4

 pcr
 1892 pT7A3E

 Nde1 + Xho1


Colony Screening

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Date: 2013-

Make miniprep and digest with Nde1 + Xho1



Result intepretation/Summary

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Conclusion

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