718-pT7A3E-wt-BamH
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Cloning of 718-pT7A3E-wt-BamH
[edit]Purpose: Cloning of 718-pT7A3E-wt-BamH
Date: 2013-12-6---2013-12-19
Notebook:0
Page:
Clone ID:718
Experimenters: yingying
Results
[edit]cloning
[edit]
Wt-BamH1-Nde1-F ctagcacatatggaagtagaaaaagagttta
Wt-BamH1-Xho1-R ttgctagctcgagtttgttttcaactttatctttc
PCR from 712 ptrc-BamHIR
| lane |
3 |
4 |
| pcr |
1892 pT7A3E | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]