1892 pT7A3E
Cloning of 1892 pT7A3E
[edit]Purpose: Cloning of 1892 pT7A3E
Date: 2013-11-1---
Notebook:0
Page:
Clone ID:1892
Experimenters: shanyi
Results
[edit]cloning
[edit]Digest vector with Xba1 + Nde1
ligate purified vector to annealed oligos:
Xba1-rbs-Nde1-T ctaga GCTCTTTAACAATTTATCA gatcca AGGAGG atatt atg aaa atc gaa ca
Xba1-rbs-Nde1-B tatgttcgattttcataatatCCTCCTtggatcTGATAAATTGTTAAAGAGCt
Colony Screening
[edit]Date: 2013-10-28
Make miniprep, digest with NcoI+EcoRV , should GET linear the vector
