1892 pT7A3E

Cloning of 1892 pT7A3E

Purpose: Cloning of 1892 pT7A3E

Date: 2013-11-1---

Notebook:0

Page:

Clone ID:1892

Experimenters:  shanyi

Results

cloning

Digest vector with Xba1 + Nde1
ligate purified vector to annealed oligos:
Xba1-rbs-Nde1-T ctaga GCTCTTTAACAATTTATCA gatcca AGGAGG atatt atg aaa atc gaa ca
Xba1-rbs-Nde1-B tatgttcgattttcataatatCCTCCTtggatcTGATAAATTGTTAAAGAGCt

Colony Screening

Date: 2013-10-28

Make miniprep, digest with NcoI+EcoRV , should GET linear the vector

•