1631 pT7A3-ID3

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Cloning of 1631 pT7A3-ID3

Purpose: Cloning of1631 pT7A3-ID3

Date: 2014-1----

Notebook:0

Page:

Clone ID:1631

Experimenters: shanyi

Results

cloning

PCR from 1260 pLenti6.2-ID3
ID3-Nde1-Nhe1-F
cgatcgCATATG GCTAGC AAGGCGCTGAGCCCGGTG
ID3-HindIII-R
tgctagAAGCTTCtAGTGGCAAAAGCTCCTTTTGTC

digest with Nde1 + HindIII, ligate to Nde1 + HindIII digested 1279 pT7A3

Colony Screening

Date: 2013-10-23

Make miniprep, digest with NdeI+HindIII

Result intepretation/Summary

Conclusion

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Cloning