1631 pT7A3-ID3
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Cloning of 1631 pT7A3-ID3
[edit]Purpose: Cloning of1631 pT7A3-ID3
Date: 2014-1----
Notebook:0
Page:
Clone ID:1631
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR from 1260 pLenti6.2-ID3
ID3-Nde1-Nhe1-F
cgatcgCATATG GCTAGC AAGGCGCTGAGCCCGGTG
ID3-HindIII-R
tgctagAAGCTTCtAGTGGCAAAAGCTCCTTTTGTC
digest with Nde1 + HindIII, ligate to Nde1 + HindIII digested 1279 pT7A3
Colony Screening
[edit]Date: 2013-10-23
Make miniprep, digest with NdeI+HindIII
