2060-pET30b-Tat-Flp-ecoli-6H

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Cloning of 2060-pET30b-Tat-Flp-ecoli-6H

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Purpose: Cloning of 2060-pET30b-Tat-Flp-ecoli-6H

Date: 2014-1-30---2014-2-3

Notebook:0

Page:

Clone ID:2060

Experimenters: yingying

Results

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cloning

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PCR using 1870 pET28b-HTNFlp-ecoli as template

yFlp-Nde1-F
Ecoli_Flp-Xho1-R


lane
 11
 12

 pcr
 pET30b

 Nde1 + Xho1



Colony Screening

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Date: 2014-2-3

Make miniprep and digest with Nde1 + Xho1


<br>

Result intepretation/Summary

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Conclusion

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