2060-pET30b-Tat-Flp-ecoli-6H
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Cloning of 2060-pET30b-Tat-Flp-ecoli-6H
[edit]Purpose: Cloning of 2060-pET30b-Tat-Flp-ecoli-6H
Date: 2014-1-30---2014-2-3
Notebook:0
Page:
Clone ID:2060
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1870 pET28b-HTNFlp-ecoli as template
yFlp-Nde1-F
Ecoli_Flp-Xho1-R
| lane |
11 |
12 |
| pcr |
pET30b | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-2-3
Make miniprep and digest with Nde1 + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]