2030-pLC6H-sfGFP

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Cloning of 2030-pLC6H-sfGFP

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Purpose: Cloning of 2030-pLC6H-sfGFP

Date: 2014-2-4---2014-2-7

Notebook:0

Page:

Clone ID:2030

Experimenters: yingying

Results

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cloning

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PCR using oligos
sfGFP-EcoR1-F

sfGFP-Not1-Xho1-6H-Pst1-R


lane
 3
 4

 pcr
 1712, pLambda-hINFA2

 EcoR1 + Pst1


Colony Screening

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Date: 2014-2-7

Make miniprep and digest with EcoR1 + Pst1



2014-2-10

digest with Bgl11+Xho1

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Result intepretation/Summary

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Conclusion

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