2030-pLC6H-sfGFP
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Cloning of 2030-pLC6H-sfGFP
[edit]Purpose: Cloning of 2030-pLC6H-sfGFP
Date: 2014-2-4---2014-2-7
Notebook:0
Page:
Clone ID:2030
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using oligos
sfGFP-EcoR1-F
sfGFP-Not1-Xho1-6H-Pst1-R
| lane |
3 |
4 |
| pcr |
1712, pLambda-hINFA2 | |
| EcoR1 + Pst1 | ||
Colony Screening
[edit]Date: 2014-2-7
Make miniprep and digest with EcoR1 + Pst1
2014-2-10
digest with Bgl11+Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]