1981-pCMV5-Tat-Flp-PDGFR
Cloning of 1981-pCMV5-Tat-Flp-PDGFR
[edit]Purpose: Cloning of 1981-pCMV5-Tat-Flp-PDGFR
Date: 2014-2-18--2014-2-20
Notebook:0
Page:
Clone ID:1981
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:PCR His-Tat-NLS from 1860 pT7A3-yFLP
6His-Nhe1-F
NLS-HindIII-R
pcr2:PCR Flpo from 1520 pDIRE
NLS-Flpo-HindIII-F
Flp-nostop-Kpn1-R
PCR:pcr1+pcr2
6His-Nhe1-F
Flp-nostop-Kpn1-R
| lane |
3 |
4 |
| pcr |
1972,1972 pCMV5-Tat-Cre-PDGFR | |
| Nhe1 + Kpn1 | ||
Colony Screening
[edit]Date: 2014-2-20
Make miniprep and digest with Hind1111 + Kpn1
