1981-pCMV5-Tat-Flp-PDGFR

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Cloning of 1981-pCMV5-Tat-Flp-PDGFR

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Purpose: Cloning of 1981-pCMV5-Tat-Flp-PDGFR

Date: 2014-2-18--2014-2-20

Notebook:0

Page:

Clone ID:1981

Experimenters: yingying

Results

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cloning

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pcr1:PCR His-Tat-NLS from 1860 pT7A3-yFLP

6His-Nhe1-F
NLS-HindIII-R

pcr2:PCR Flpo from 1520 pDIRE

NLS-Flpo-HindIII-F

Flp-nostop-Kpn1-R


PCR:pcr1+pcr2

6His-Nhe1-F
Flp-nostop-Kpn1-R


lane
 3
 4

 pcr
 1972,1972 pCMV5-Tat-Cre-PDGFR

 Nhe1 + Kpn1


Colony Screening

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Date: 2014-2-20

Make miniprep and digest with  Hind1111 + Kpn1



Result intepretation/Summary

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Conclusion

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