1940-pLysN-ha-Plac-Hia5

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Cloning of 1940-pLysN-ha-Plac-Hia5

[edit]

Purpose: Cloning of 1940-pLysN-ha-Plac-Hia5

Date: 2014-1-8---2014-1-13

Notebook:0

Page:

Clone ID:1940

Experimenters: yingying

Results

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cloning

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1838 pEC-hia5
ha-constitutive-PLac-F
ha-constitutive-PLac-Hia5-F


lane
 5
 6

 pcr
 674 pLysN-Plac-mXho1

     HindIII + Xho1


Colony Screening

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Date: 2014-1-13

Make miniprep and digest with HindIII + Xho1


<br>

Result intepretation/Summary

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Conclusion

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subCloning of 1940-pLysN-ha-Plac-Hia5

[edit]

Purpose: Cloning of 1940-pLysN-ha-Plac-Hia5

Date: 2014-2-12---2014-2-14

Notebook:0

Page:

Clone ID:1940

Experimenters: yingying

Results

[edit]

cloning

[edit]


lane
 5
 6

 2069,pEC-ha-Plac-hia5
 674 pLysN-Plac-mXho1

     HindIII + Xho1


Colony Screening

[edit]

Date: 2014-2-14

Make miniprep and digest with HindIII + Xho1


c:674 pLysN-Plac-mXho1


digest with HindIII +BamH1


2014-2-21

PCR Screen:pLysN-HindIII-seq-F2
Hia5-Xho1-F


c:674 pLysN-Plac-mXho1

<br>

Result intepretation/Summary

[edit]

Conclusion

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