1940-pLysN-ha-Plac-Hia5
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Cloning of 1940-pLysN-ha-Plac-Hia5
[edit]Purpose: Cloning of 1940-pLysN-ha-Plac-Hia5
Date: 2014-1-8---2014-1-13
Notebook:0
Page:
Clone ID:1940
Experimenters: yingying
Results
[edit]cloning
[edit]
1838 pEC-hia5
ha-constitutive-PLac-F
ha-constitutive-PLac-Hia5-F
| lane |
5 |
6 |
| pcr |
674 pLysN-Plac-mXho1 | |
| HindIII + Xho1 | ||
Colony Screening
[edit]Date: 2014-1-13
Make miniprep and digest with HindIII + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
subCloning of 1940-pLysN-ha-Plac-Hia5
[edit]Purpose: Cloning of 1940-pLysN-ha-Plac-Hia5
Date: 2014-2-12---2014-2-14
Notebook:0
Page:
Clone ID:1940
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
5 |
6 |
| 2069,pEC-ha-Plac-hia5 |
674 pLysN-Plac-mXho1 | |
| HindIII + Xho1 | ||
Colony Screening
[edit]Date: 2014-2-14
Make miniprep and digest with HindIII + Xho1
c:674 pLysN-Plac-mXho1
digest with HindIII +BamH1
2014-2-21
PCR Screen:pLysN-HindIII-seq-F2
Hia5-Xho1-F
c:674 pLysN-Plac-mXho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]