2100 pFastBac-Dual-VSVG

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Cloning of 2100 pFastBac-Dual-VSVG

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Purpose: Cloning of2100 pFastBac-Dual-VSVG==

Date: 2014-2----

Notebook:0

Page:

Clone ID:2100

Experimenters:  shanyi

Results

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CLONING

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amplify from VSVG plasmid 505 pCMV-VSVG
VSVG-Xho1-F
tagca ctcgag gccacc ATGAAGTGCCTTTTGTACTTAG

VSVG-Nhe1-R
(GATGAACCGACTTGGAAAGTAA GCT AGC aagcaa)
ttgcttGCTAGCTTACTTTCCAAGTCGGTTCATC


digest with Xho1-Nhe1


Colony Screening

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Date: 2014

Make miniprep, digest with Xho1-Nhe1

sent 2100-1 to sequence with p10-Pro-seq-F



Result intepretation/Summary

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Conclusion

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