2100 pFastBac-Dual-VSVG
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Cloning of 2100 pFastBac-Dual-VSVG
[edit]Purpose: Cloning of2100 pFastBac-Dual-VSVG==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2100
Experimenters: shanyi
Results
[edit]CLONING
[edit]amplify from VSVG plasmid 505 pCMV-VSVG
VSVG-Xho1-F
tagca ctcgag gccacc ATGAAGTGCCTTTTGTACTTAG
VSVG-Nhe1-R
(GATGAACCGACTTGGAAAGTAA GCT AGC aagcaa)
ttgcttGCTAGCTTACTTTCCAAGTCGGTTCATC
digest with Xho1-Nhe1
Colony Screening
[edit]Date: 2014
Make miniprep, digest with Xho1-Nhe1
sent 2100-1 to sequence with p10-Pro-seq-F