2050 pT7A3E-CeBD-6H

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Cloning of 2050 pT7A3E-CeBD-6H

Purpose: Cloning of 2050 pT7A3E-CeBD-6H==

Date: 2014-2----

Notebook:0

Page:

Clone ID:2050

Experimenters: shanyi

Results

CLONING

Gene synthesis

digestPCR with HindIII + Sal1 , digest vector 1982 with HindIII +Xho1

Colony Screening

Date: 2014

Make miniprep, digest with HindIII + EcoRV-->1400+540=1940 bp,

use Kpn1 + EcoRV , there is no KpnI site in 1892

Result intepretation/Summary

Conclusion

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Cloning