2050 pT7A3E-CeBD-6H
Jump to navigation
Jump to search
Cloning of 2050 pT7A3E-CeBD-6H
[edit]Purpose: Cloning of 2050 pT7A3E-CeBD-6H==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2050
Experimenters: shanyi
Results
[edit]CLONING
[edit]Gene synthesis
digestPCR with HindIII + Sal1 , digest vector 1982 with HindIII +Xho1
Colony Screening
[edit]Date: 2014
Make miniprep, digest with HindIII + EcoRV-->1400+540=1940 bp,
use Kpn1 + EcoRV , there is no KpnI site in 1892
