2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A

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Cloning of 2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A

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Purpose: Cloning of2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A==

Date: 2014-2----

Notebook:0

Page:

Clone ID:2068

Experimenters:  shanyi

Results

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CLONING

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PCR NpuDnaE-N from 1742 p8H-ELK-IN
GS-NpuDnaE-N-Nde1-F
atcatc cat atg ggaggaggtgggtctggaggtggaggatct GCg TTAAGCTATGAAACGGAAATATTG
Xho1
NpuDnaE-N-12 TGTACGCTCGAGTTAATTCGGCAAATTATCAACCCGCATCAAATCC



digest with Nde1 + XhoI clone to 2067 pT7A3-6H-CBD



Colony Screening

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Date: 2014

Make miniprep, digest with Nde1 + XhoI



Result intepretation/Summary

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Conclusion

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