2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A
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Cloning of 2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A
[edit]Purpose: Cloning of2068 pT7A3-6H-ChiBD-N-NpuDnaE-C1A==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2068
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR NpuDnaE-N from 1742 p8H-ELK-IN
GS-NpuDnaE-N-Nde1-F
atcatc cat atg ggaggaggtgggtctggaggtggaggatct GCg TTAAGCTATGAAACGGAAATATTG
Xho1
NpuDnaE-N-12 TGTACGCTCGAGTTAATTCGGCAAATTATCAACCCGCATCAAATCC
digest with Nde1 + XhoI clone to 2067 pT7A3-6H-CBD
Colony Screening
[edit]Date: 2014
Make miniprep, digest with Nde1 + XhoI
