FLP VLP Clone Comparison

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Date:____Transfection: Gag-FLP clones with or without VSVG transfect 293T cells, 12-well plate

Date:____Use condition media 0.5 ml to transduce 293T transfected with FRT reporter 2013 pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP, 24 well plate

1445 +vsvg

pCMV5-Gag-NLS-Flp-WPRE-oriP, HIV Gag

1969 + VSVG

pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP, HIV Gag

2103 + VSVG

pCMV-RSV-Gag-Flpo, RSV-Gag-Flpo

2109 + VSVG

pCMV-RSV-Gag-Flpe, RSV-Gag-Flpe

1445, pCMV5-Gag-NLS-Flp-WPRE-oriP, HIV Gag
 1969, pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP, HIV Gag
 2103, pCMV-RSV-Gag-Flpo, RSV-Gag-Flpo
 2109, pCMV-RSV-Gag-Flpe, RSV-Gag-Flpe




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