FLP VLP Clone Comparison
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Date:____Transfection: Gag-FLP clones with or without VSVG transfect 293T cells, 12-well plate
Date:____Use condition media 0.5 ml to transduce 293T transfected with FRT reporter 2013 pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP, 24 well plate
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1445 +vsvg pCMV5-Gag-NLS-Flp-WPRE-oriP, HIV Gag |
1969 + VSVG pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP, HIV Gag |
2103 + VSVG pCMV-RSV-Gag-Flpo, RSV-Gag-Flpo |
2109 + VSVG pCMV-RSV-Gag-Flpe, RSV-Gag-Flpe |
| 1445, pCMV5-Gag-NLS-Flp-WPRE-oriP, HIV Gag |
1969, pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP, HIV Gag |
2103, pCMV-RSV-Gag-Flpo, RSV-Gag-Flpo |
2109, pCMV-RSV-Gag-Flpe, RSV-Gag-Flpe |
