1882-pLysN-Plac-Hia5

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Cloning of 1882-pLysN-Plac-Hia5

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Purpose: Cloning of 1882-pLysN-Plac-Hia5

Date: 2014-3-24---2014-3-27

Notebook:0

Page:

Clone ID:1882

Experimenters: yingying

Results

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cloning

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PCR using these two oligos using 1838 pEC-hia5
Hia5-Nde1-F

Hia5-Xho1-F


lane
 1
 2

 pcr
 674pLysN-mNhe1

 Nde1 + Xho1


Colony Screening

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Date: 2014-3-27

Make miniprep and digest with Nde1 + Xho1


<br>

Result intepretation/Summary

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Conclusion

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