1882-pLysN-Plac-Hia5
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Cloning of 1882-pLysN-Plac-Hia5
[edit]Purpose: Cloning of 1882-pLysN-Plac-Hia5
Date: 2014-3-24---2014-3-27
Notebook:0
Page:
Clone ID:1882
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using these two oligos using 1838 pEC-hia5
Hia5-Nde1-F
Hia5-Xho1-F
| lane |
1 |
2 |
| pcr |
674pLysN-mNhe1 | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-3-27
Make miniprep and digest with Nde1 + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]