2155-pEC2-6H-tat-sc-yMS2

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Cloning of 2155-pEC2-6H-tat-sc-yMS2

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Purpose: Cloning of 2155-pEC2-6H-tat-sc-yMS2

Date: 2014-4-4---2014-4-9

Notebook:0

Page:

Clone ID:2155

Experimenters: yingying

Results

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cloning

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 pcr1:gene synthesis yMS2-1 to  yMS2-12

pcr2: gene synthesis GLink-MS2-F to  YMS2-EcoR1-R


pcr1+pcr2
yMS2-1
YMS2-EcoR1-R


lane
 1
 2

 PCR
 2015 pEC2

 Nde1 + EcoR1


Colony Screening

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Date: 2014-4-9

Make miniprep and digest with Nde1 + EcoR1


<br>

Result intepretation/Summary

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Conclusion

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