2155-pEC2-6H-tat-sc-yMS2
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Cloning of 2155-pEC2-6H-tat-sc-yMS2
[edit]Purpose: Cloning of 2155-pEC2-6H-tat-sc-yMS2
Date: 2014-4-4---2014-4-9
Notebook:0
Page:
Clone ID:2155
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:gene synthesis yMS2-1 to yMS2-12
pcr2: gene synthesis GLink-MS2-F to YMS2-EcoR1-R
pcr1+pcr2
yMS2-1
YMS2-EcoR1-R
| lane |
1 |
2 |
| PCR |
2015 pEC2 | |
| Nde1 + EcoR1 | ||
Colony Screening
[edit]Date: 2014-4-9
Make miniprep and digest with Nde1 + EcoR1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]