2146-pT7A3HC-6H-Tat-sc-MS2

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Cloning of 2146-pT7A3HC-6H-Tat-sc-MS2

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Purpose: Cloning of 2146-pT7A3HC-6H-Tat-sc-MS2

Date: 2014-4-14---2014

Notebook:0

Page:

Clone ID:2146

Experimenters: yingying

Results

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cloning

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pcr1 MS2 fragment to amplify MS2 from 2145 pT7A3HC-6H-Tat-MS2
MS2-linker-F
MS2-EcoR1-Term-R


pcr2: same with 2148pcr2

T7 promoter from 706 pRsetA
Term-T7-F
T7-HindIII-R


2014-4-17


PCR:pcr1+pcr2

MS2-linker-F
T7-HindIII-R

lane
 1
 2
 3

 PCR
 PCR
 2145, pT7A3HC-6H-Tat-MS2

 BamH1 + HindIII




Colony Screening

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Date: 2014-4-17

Make miniprep and digest with  BamH1+Hind111



Result intepretation/Summary

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Conclusion

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