2146-pT7A3HC-6H-Tat-sc-MS2
Jump to navigation
Jump to search
Cloning of 2146-pT7A3HC-6H-Tat-sc-MS2
[edit]Purpose: Cloning of 2146-pT7A3HC-6H-Tat-sc-MS2
Date: 2014-4-14---2014
Notebook:0
Page:
Clone ID:2146
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1 MS2 fragment to amplify MS2 from 2145 pT7A3HC-6H-Tat-MS2
MS2-linker-F
MS2-EcoR1-Term-R
pcr2: same with 2148pcr2
T7 promoter from 706 pRsetA
Term-T7-F
T7-HindIII-R
2014-4-17
PCR:pcr1+pcr2
MS2-linker-F
T7-HindIII-R
| lane |
1 |
2 |
3 |
| PCR |
PCR |
2145, pT7A3HC-6H-Tat-MS2 | |
| BamH1 + HindIII | |||
Colony Screening
[edit]Date: 2014-4-17
Make miniprep and digest with BamH1+Hind111
