2142-pCMV5-p53-wt

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Revision as of 14:43, 23 April 2014 by Paul.xu (talk | contribs) (Created page with "== '''Cloning of 2142-pCMV5-p53-wt''' == '''Purpose:''' Cloning of 1519-pT7A3-T3-DNA-Ligase '''Date: '''2014-4-21---2014-4-23 '''Notebook:0''' '''Page:''' '''Clone ID:2...")
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Cloning of 2142-pCMV5-p53-wt

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Purpose: Cloning of 1519-pT7A3-T3-DNA-Ligase

Date: 2014-4-21---2014-4-23

Notebook:0

Page:

Clone ID:2142

Experimenters: yingying

Results

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cloning

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PCR from 80 pLex-p53wt
p53-wt-BamH1-F
p53-wt-EcoR1-R


lane
 3
 4

 pcr
 1973 pCMV5-CD16-RIgE

 BamH1 + EcoR1


Colony Screening

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Date: 2014-4-23

Make miniprep and digest with BamH1 + EcoR1


<br>

Result intepretation/Summary

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Conclusion

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