2142-pCMV5-p53-wt
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Cloning of 2142-pCMV5-p53-wt
[edit]Purpose: Cloning of 1519-pT7A3-T3-DNA-Ligase
Date: 2014-4-21---2014-4-23
Notebook:0
Page:
Clone ID:2142
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR from 80 pLex-p53wt
p53-wt-BamH1-F
p53-wt-EcoR1-R
| lane |
3 |
4 |
| pcr |
1973 pCMV5-CD16-RIgE | |
| BamH1 + EcoR1 | ||
Colony Screening
[edit]Date: 2014-4-23
Make miniprep and digest with BamH1 + EcoR1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]